CXCL14
Gene Ontology Biological Process
Gene Ontology Cellular Component
CCL5
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- MAPK cascade [IMP]
- activation of phospholipase D activity [IDA]
- calcium ion transport [IDA]
- cell-cell signaling [IDA]
- cellular calcium ion homeostasis [IDA]
- cellular protein complex assembly [IDA]
- cellular response to fibroblast growth factor stimulus [IEP]
- cellular response to interferon-gamma [IEP]
- cellular response to interleukin-1 [IEP]
- cellular response to organic cyclic compound [IDA]
- cellular response to tumor necrosis factor [IEP]
- chemokine-mediated signaling pathway [IDA, TAS]
- chemotaxis [NAS]
- dendritic cell chemotaxis [TAS]
- eosinophil chemotaxis [IDA]
- exocytosis [IDA]
- inflammatory response [IDA]
- leukocyte cell-cell adhesion [IDA]
- lipopolysaccharide-mediated signaling pathway [IDA]
- macrophage chemotaxis [TAS]
- monocyte chemotaxis [IC]
- negative regulation by host of viral transcription [IDA]
- negative regulation of G-protein coupled receptor protein signaling pathway [IDA]
- negative regulation of T cell apoptotic process [IDA]
- negative regulation of chemokine-mediated signaling pathway [IDA]
- negative regulation of viral genome replication [IDA]
- neutrophil activation [IDA]
- positive chemotaxis [IDA]
- positive regulation of JAK-STAT cascade [TAS]
- positive regulation of T cell apoptotic process [IDA]
- positive regulation of T cell chemotaxis [IDA]
- positive regulation of T cell migration [IDA]
- positive regulation of T cell proliferation [IDA]
- positive regulation of activation of JAK2 kinase activity [TAS]
- positive regulation of calcium ion transport [IDA]
- positive regulation of cell adhesion [IDA]
- positive regulation of cell migration [IDA]
- positive regulation of cell-cell adhesion mediated by integrin [IDA]
- positive regulation of cellular biosynthetic process [IDA]
- positive regulation of homotypic cell-cell adhesion [IDA]
- positive regulation of innate immune response [TAS]
- positive regulation of macrophage chemotaxis [IDA]
- positive regulation of monocyte chemotaxis [IDA]
- positive regulation of natural killer cell chemotaxis [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of phosphorylation [IDA]
- positive regulation of protein tyrosine kinase activity [IDA]
- positive regulation of smooth muscle cell migration [IDA]
- positive regulation of smooth muscle cell proliferation [IDA]
- positive regulation of translational initiation [NAS]
- positive regulation of tyrosine phosphorylation of STAT protein [IDA]
- positive regulation of viral genome replication [TAS]
- protein kinase B signaling [IMP]
- protein phosphorylation [IDA]
- protein tetramerization [IDA]
- regulation of T cell activation [IDA]
- regulation of chronic inflammatory response [TAS]
- regulation of insulin secretion [IDA]
- regulation of neuron death [IDA]
- response to toxic substance [IDA]
- response to virus [TAS]
Gene Ontology Molecular Function- CCR1 chemokine receptor binding [IDA, IPI, TAS]
- CCR4 chemokine receptor binding [TAS]
- CCR5 chemokine receptor binding [IPI]
- chemoattractant activity [IDA]
- chemokine activity [IDA, NAS]
- chemokine receptor antagonist activity [IDA]
- chemokine receptor binding [IPI]
- phosphatidylinositol phospholipase C activity [IDA]
- phospholipase activator activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase activity [IDA]
- protein self-association [IDA]
- receptor signaling protein tyrosine kinase activator activity [IDA]
- CCR1 chemokine receptor binding [IDA, IPI, TAS]
- CCR4 chemokine receptor binding [TAS]
- CCR5 chemokine receptor binding [IPI]
- chemoattractant activity [IDA]
- chemokine activity [IDA, NAS]
- chemokine receptor antagonist activity [IDA]
- chemokine receptor binding [IPI]
- phosphatidylinositol phospholipase C activity [IDA]
- phospholipase activator activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase activity [IDA]
- protein self-association [IDA]
- receptor signaling protein tyrosine kinase activator activity [IDA]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
Chemokines orchestrate leukocyte trafficking and function in health and disease. Heterophilic interactions between chemokines in a given microenvironment may amplify, inhibit, or modulate their activity; however, a systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting and surface plasmon resonance to obtain a comprehensive map of chemokine-chemokine interactions and to confirm their specificity. Structure-function analyses ... [more]
Quantitative Score
- 0.238 [Relative OD]
Throughput
- High Throughput
Additional Notes
- averages of 2-4 experiments were used
- interaction assayed using densitometry analysis of bidirectional immunoligand blotting in which the bait protein was immobilized on a membrane, the prey protein was added in solution, prey was detected by specific antibody, and the densitometric optical density (OD) was normalized for area and reported as a ratio of a corresponding positive control
- interactions were considered positive if this relative ratio was equal to or greater than 0.05
Curated By
- BioGRID