PF4V1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CCL19
Gene Ontology Biological Process
- T cell costimulation [ISS]
- activation of JUN kinase activity [ISS]
- cell communication [TAS]
- cell maturation [ISS]
- cellular calcium ion homeostasis [TAS]
- dendritic cell chemotaxis [IDA]
- establishment of T cell polarity [IDA]
- immunological synapse formation [ISS]
- inflammatory response [NAS]
- interleukin-12 secretion [ISS]
- mature conventional dendritic cell differentiation [ISS]
- myeloid dendritic cell chemotaxis [IDA]
- negative regulation of leukocyte apoptotic process [IDA]
- positive regulation of Cdc42 GTPase activity [ISS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of JNK cascade [IDA]
- positive regulation of NF-kappaB import into nucleus [IDA]
- positive regulation of Ras GTPase activity [ISS]
- positive regulation of T cell proliferation [ISS]
- positive regulation of T-helper 1 cell differentiation [ISS]
- positive regulation of cell motility [IDA]
- positive regulation of chemotaxis [ISS]
- positive regulation of dendritic cell antigen processing and presentation [ISS]
- positive regulation of dendritic cell dendrite assembly [ISS]
- positive regulation of endocytosis [ISS]
- positive regulation of glycoprotein biosynthetic process [ISS]
- positive regulation of interleukin-1 beta secretion [ISS]
- positive regulation of interleukin-12 production [ISS]
- positive regulation of neutrophil chemotaxis [IDA]
- positive regulation of phosphatidylinositol 3-kinase activity [IDA]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of protein kinase activity [IDA]
- positive regulation of receptor-mediated endocytosis [ISS]
- positive regulation of tumor necrosis factor production [ISS]
- regulation of cell projection assembly [ISS]
- release of sequestered calcium ion into cytosol [IDA]
- response to nitric oxide [IDA]
- response to prostaglandin E [IDA]
- response to virus [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
Chemokines orchestrate leukocyte trafficking and function in health and disease. Heterophilic interactions between chemokines in a given microenvironment may amplify, inhibit, or modulate their activity; however, a systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting and surface plasmon resonance to obtain a comprehensive map of chemokine-chemokine interactions and to confirm their specificity. Structure-function analyses ... [more]
Quantitative Score
- 0.593 [Relative OD]
Throughput
- High Throughput
Additional Notes
- averages of 2-4 experiments were used
- interaction assayed using densitometry analysis of bidirectional immunoligand blotting in which the bait protein was immobilized on a membrane, the prey protein was added in solution, prey was detected by specific antibody, and the densitometric optical density (OD) was normalized for area and reported as a ratio of a corresponding positive control
- interactions were considered positive if this relative ratio was equal to or greater than 0.05
Curated By
- BioGRID