PF4V1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CXCL13
Gene Ontology Biological Process
- B cell chemotaxis [IDA]
- G-protein coupled receptor signaling pathway [IBA]
- activation of Rap GTPase activity [ISS]
- cell surface receptor signaling pathway [IDA]
- cell-cell signaling [IDA]
- chemokine-mediated signaling pathway [IBA]
- chronic inflammatory response [NAS]
- defense response to bacterium [IDA]
- endothelial cell chemotaxis to fibroblast growth factor [IDA]
- germinal center formation [NAS]
- immune response [IBA, IC]
- inflammatory response [IBA]
- lymphocyte chemotaxis across high endothelial venule [ISS]
- negative regulation of endothelial cell chemotaxis to fibroblast growth factor [IDA]
- positive regulation of T cell chemotaxis [IDA]
- positive regulation of cell-cell adhesion mediated by integrin [ISS]
- positive regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of integrin activation [ISS]
- regulation of angiogenesis [NAS]
- regulation of cell proliferation [IBA]
- regulation of humoral immune response [NAS]
- response to lipopolysaccharide [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
Chemokines orchestrate leukocyte trafficking and function in health and disease. Heterophilic interactions between chemokines in a given microenvironment may amplify, inhibit, or modulate their activity; however, a systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting and surface plasmon resonance to obtain a comprehensive map of chemokine-chemokine interactions and to confirm their specificity. Structure-function analyses ... [more]
Quantitative Score
- 0.419 [Relative OD]
Throughput
- High Throughput
Additional Notes
- averages of 2-4 experiments were used
- interaction assayed using densitometry analysis of bidirectional immunoligand blotting in which the bait protein was immobilized on a membrane, the prey protein was added in solution, prey was detected by specific antibody, and the densitometric optical density (OD) was normalized for area and reported as a ratio of a corresponding positive control
- interactions were considered positive if this relative ratio was equal to or greater than 0.05
Curated By
- BioGRID