BAIT
PRKCD
ALPS3, CVID9, MAY1, PKCD, nPKC-delta
protein kinase C, delta
GO Process (41)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- RNA metabolic process [TAS]
- activation of phospholipase C activity [TAS]
- apoptotic process [IDA, TAS]
- blood coagulation [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular senescence [IMP]
- cytokine-mediated signaling pathway [TAS]
- defense response to bacterium [ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- intrinsic apoptotic signaling pathway in response to oxidative stress [TAS]
- mRNA metabolic process [TAS]
- negative regulation of MAP kinase activity [IMP]
- negative regulation of actin filament polymerization [ISS]
- negative regulation of filopodium assembly [ISS]
- negative regulation of glial cell apoptotic process [IMP]
- negative regulation of inflammatory response [IC]
- negative regulation of insulin receptor signaling pathway [ISS, TAS]
- negative regulation of peptidyl-tyrosine phosphorylation [ISS]
- negative regulation of platelet aggregation [ISS]
- negative regulation of protein binding [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- neutrophil activation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- platelet activation [TAS]
- positive regulation of ceramide biosynthetic process [IMP]
- positive regulation of glucosylceramide catabolic process [IMP]
- positive regulation of phospholipid scramblase activity [IMP]
- positive regulation of protein dephosphorylation [IMP]
- positive regulation of response to DNA damage stimulus [IMP]
- positive regulation of sphingomyelin catabolic process [IMP]
- positive regulation of superoxide anion generation [IMP]
- protein phosphorylation [IDA]
- protein stabilization [NAS]
- regulation of receptor activity [TAS]
- signal transduction [TAS]
- termination of signal transduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PTK2B
CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK
protein tyrosine kinase 2 beta
GO Process (53)
GO Function (7)
GO Component (15)
Gene Ontology Biological Process
- activation of Janus kinase activity [IMP]
- angiogenesis [IBA]
- apoptotic process [TAS]
- bone resorption [ISS]
- cell surface receptor signaling pathway [IMP]
- cellular defense response [ISS]
- cellular response to retinoic acid [IMP]
- chemokine-mediated signaling pathway [ISS]
- epidermal growth factor receptor signaling pathway [IBA]
- innate immune response [IBA]
- integrin-mediated signaling pathway [IMP]
- ionotropic glutamate receptor signaling pathway [ISS]
- long-term synaptic potentiation [ISS]
- marginal zone B cell differentiation [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of bone mineralization [ISS]
- negative regulation of cell proliferation [IMP]
- negative regulation of myeloid cell differentiation [IMP]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of potassium ion transport [IDA]
- peptidyl-tyrosine autophosphorylation [IBA, ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of B cell chemotaxis [ISS]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of JNK cascade [IMP]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cell-matrix adhesion [IMP]
- positive regulation of endothelial cell migration [IDA]
- positive regulation of excitatory postsynaptic membrane potential [ISS]
- positive regulation of neuron projection development [IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of phosphatidylinositol 3-kinase activity [ISS]
- positive regulation of protein kinase activity [IMP]
- positive regulation of synaptic transmission, glutamatergic [ISS]
- protein autophosphorylation [TAS]
- protein complex assembly [TAS]
- protein phosphorylation [TAS]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISS]
- regulation of actin cytoskeleton reorganization [ISS]
- regulation of cell adhesion [IMP]
- regulation of cell shape [IMP]
- regulation of establishment of cell polarity [ISS]
- regulation of inositol trisphosphate biosynthetic process [ISS]
- regulation of macrophage chemotaxis [ISS]
- regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of release of sequestered calcium ion into cytosol [ISS]
- response to stress [TAS]
- signal transduction [TAS]
- sprouting angiogenesis [ISS]
- tumor necrosis factor-mediated signaling pathway [IMP]
- vascular endothelial growth factor receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [ISS]
- apical dendrite [ISS]
- cell body [ISS]
- cytoplasm [IDA]
- cytosol [TAS]
- dendrite [ISS]
- extrinsic component of cytoplasmic side of plasma membrane [IBA]
- focal adhesion [IDA]
- growth cone [ISS]
- lamellipodium [IDA]
- neuronal cell body [ISS]
- nucleoplasm [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- postsynaptic density [ISS]
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Carbachol stimulates TYR phosphorylation and association of PKCdelta and PYK2 in pancreas.
Carbachol treatment resulted in increased phosphorylation on tyrosine of PKCdelta immunoprecipitated from rat pancreatic acinar cells. The Ca2+-dependent tyrosine kinase PYK2 coimmunoprecipitated with PKCdelta from carbachol-exposed cells and also exhibited increased tyrosine phosphorylation. Tyrosine phosphorylation of both PKCdelta and PYK2 was concentration-dependent with respect to carbachol, and rapid, reaching maximal levels by 5 min of treatment. Exposure of acinar cells ... [more]
Biochem. Biophys. Res. Commun. Apr. 13, 2001; 282(4);882-6 [Pubmed: 11352632]
Throughput
- Low Throughput
Curated By
- BioGRID