BAIT
FASN
FAS, OA-519, SDR27X1
fatty acid synthase
GO Process (11)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- fatty acid metabolic process [TAS]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- osteoblast differentiation [IDA]
- pantothenate metabolic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
INPP5K
PPS, SKIP
inositol polyphosphate-5-phosphatase K
GO Process (37)
GO Function (8)
GO Component (11)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS]
- actin cytoskeleton organization [NAS]
- cellular response to cAMP [ISS]
- cellular response to epidermal growth factor stimulus [IDA]
- cellular response to hormone stimulus [ISS]
- cellular response to insulin stimulus [IDA]
- cellular response to tumor necrosis factor [IDA]
- dephosphorylation [IDA, IMP]
- glucose homeostasis [ISS]
- inositol phosphate dephosphorylation [IDA]
- negative regulation by host of viral transcription [IDA]
- negative regulation of MAP kinase activity [IDA]
- negative regulation of calcium ion transport [IDA]
- negative regulation of dephosphorylation [ISS]
- negative regulation of glucose transport [IDA]
- negative regulation of glycogen (starch) synthase activity [ISS]
- negative regulation of glycogen biosynthetic process [IDA]
- negative regulation of insulin receptor signaling pathway [IDA]
- negative regulation of peptidyl-serine phosphorylation [IDA]
- negative regulation of peptidyl-threonine phosphorylation [IDA]
- negative regulation of protein kinase B signaling [IDA]
- negative regulation of protein kinase activity [IDA]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of protein targeting to membrane [IDA]
- negative regulation of single stranded viral RNA replication via double stranded DNA intermediate [IDA]
- negative regulation of stress fiber assembly [IDA]
- negative regulation of transcription, DNA-templated [ISS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol dephosphorylation [IDA]
- phospholipid metabolic process [TAS]
- positive regulation of renal water transport [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of urine volume [ISS]
- protein targeting to plasma membrane [ISS]
- regulation of glycogen biosynthetic process [ISS]
- ruffle assembly [IDA]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- inositol bisphosphate phosphatase activity [IDA]
- inositol trisphosphate phosphatase activity [IDA]
- lipid phosphatase activity [NAS]
- phosphatidylinositol phosphate 5-phosphatase activity [IMP]
- phosphatidylinositol trisphosphate phosphatase activity [IDA]
- phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity [IDA]
- protein binding [IPI]
- vasopressin receptor activity [ISS]
- inositol bisphosphate phosphatase activity [IDA]
- inositol trisphosphate phosphatase activity [IDA]
- lipid phosphatase activity [NAS]
- phosphatidylinositol phosphate 5-phosphatase activity [IMP]
- phosphatidylinositol trisphosphate phosphatase activity [IDA]
- phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity [IDA]
- protein binding [IPI]
- vasopressin receptor activity [ISS]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID