BAIT
FASN
FAS, OA-519, SDR27X1
fatty acid synthase
GO Process (11)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- fatty acid metabolic process [TAS]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- osteoblast differentiation [IDA]
- pantothenate metabolic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PAXIP1
CAGF29, PACIP1, PAXIP1L, PTIP, TNRC2, CAGF28
PAX interacting (with transcription-activation domain) protein 1
GO Process (9)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator [IMP]
- histone H3-K4 methylation [IDA]
- positive regulation of histone H3-K36 methylation [ISS]
- positive regulation of histone H3-K4 methylation [ISS]
- positive regulation of histone acetylation [ISS]
- positive regulation of isotype switching [ISS]
- positive regulation of protein ubiquitination [ISS]
- positive regulation of transcription initiation from RNA polymerase II promoter [IMP, ISS]
- response to ionizing radiation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID