BAIT
FASN
FAS, OA-519, SDR27X1
fatty acid synthase
GO Process (11)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- fatty acid metabolic process [TAS]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- osteoblast differentiation [IDA]
- pantothenate metabolic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SOX4
EVI16
SRY (sex determining region Y)-box 4
GO Process (41)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [IDA]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IMP]
- T cell differentiation [ISS]
- ascending aorta morphogenesis [ISS]
- atrial septum primum morphogenesis [ISS]
- canonical Wnt signaling pathway [ISS]
- cardiac right ventricle morphogenesis [ISS]
- cardiac ventricle formation [ISS]
- cellular response to glucose stimulus [ISS]
- glial cell development [ISS]
- glial cell proliferation [ISS]
- glucose homeostasis [ISS]
- heart development [ISS]
- kidney morphogenesis [ISS]
- limb bud formation [ISS]
- mitral valve morphogenesis [ISS]
- negative regulation of cell death [ISS]
- negative regulation of cell proliferation [IMP]
- negative regulation of protein export from nucleus [IMP]
- negative regulation of protein ubiquitination [IMP]
- neural tube formation [ISS]
- neuroepithelial cell differentiation [ISS]
- noradrenergic neuron differentiation [ISS]
- positive regulation of N-terminal peptidyl-lysine acetylation [IDA]
- positive regulation of Wnt signaling pathway [ISS]
- positive regulation of apoptotic process [IMP]
- positive regulation of canonical Wnt signaling pathway [ISS]
- positive regulation of cell proliferation [IMP]
- positive regulation of insulin secretion [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of translation [IMP]
- pro-B cell differentiation [ISS]
- protein stabilization [IMP]
- regulation of protein stability [IMP]
- regulation of transcription, DNA-templated [IDA]
- skeletal system development [ISS]
- spinal cord development [ISS]
- spinal cord motor neuron differentiation [ISS]
- sympathetic nervous system development [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISS]
- core promoter sequence-specific DNA binding [IDA]
- nucleic acid binding transcription factor activity [IMP]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISS]
- core promoter sequence-specific DNA binding [IDA]
- nucleic acid binding transcription factor activity [IMP]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID