BAIT
FASN
FAS, OA-519, SDR27X1
fatty acid synthase
GO Process (11)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- fatty acid metabolic process [TAS]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- osteoblast differentiation [IDA]
- pantothenate metabolic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATF4
CREB-2, CREB2, TAXREB67, TXREB
activating transcription factor 4
GO Process (20)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cellular amino acid metabolic process [TAS]
- cellular protein metabolic process [TAS]
- cellular response to glucose starvation [IMP]
- circadian regulation of gene expression [ISS]
- endoplasmic reticulum unfolded protein response [TAS]
- gluconeogenesis [ISS]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISS]
- negative regulation of oxidative stress-induced neuron death [IGI]
- positive regulation of apoptotic process [TAS]
- positive regulation of neuron apoptotic process [ISS]
- positive regulation of transcription from RNA polymerase I promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress [IMP, ISS]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISS, NAS]
- positive regulation vascular endothelial growth factor production [IMP]
- regulation of transcription, DNA-templated [ISS]
- response to endoplasmic reticulum stress [IDA]
- transcription from RNA polymerase II promoter [ISS]
Gene Ontology Molecular Function- DNA binding [IDA, ISS]
- core promoter sequence-specific DNA binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IC]
- sequence-specific DNA binding transcription factor activity [ISS, TAS]
- transcription regulatory region DNA binding [IDA, TAS]
- DNA binding [IDA, ISS]
- core promoter sequence-specific DNA binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IC]
- sequence-specific DNA binding transcription factor activity [ISS, TAS]
- transcription regulatory region DNA binding [IDA, TAS]
Gene Ontology Cellular Component
Homo sapiens
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic positive genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID