An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The cell proliferation antigen Ki-67 organises heterochromatin.

Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Lleres D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Dejardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D

Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in ... [more]

Elife Mar. 07, 2016; 5();e13722 [Pubmed: 26949251]

Quantitative Score

137.1 [Mascot Score]

Throughput

High Throughput

Additional Notes

interactors identified using mass spectrometry, retaining hits for which either an untagged immunoprecipitation (IP) or IP of non-specific protein (TRIM39) produced fewer peptides hits and/or lower Mascot Scores

Curated By

BioGRID

Interaction Summary

MKI67

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

MARK2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]magnesium ion binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase activator activity [TAS]protein serine/threonine kinase activity [IDA, NAS, TAS]tau-protein kinase activity [ISS]