BAIT

MKI67

KIA, MIB-, MIB-1, PPP1R105, RP11-380J17.2
marker of proliferation Ki-67
Glioblastoma Project
GO Process (1)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SLC25A6

2, 3, AAC3, ANT, ANT 2, ANT 3, ANT3, ANT3Y, RP11-261P4.6
solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The cell proliferation antigen Ki-67 organises heterochromatin.

Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Lleres D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Dejardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D

Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in ... [more]

Elife Mar. 07, 2016; 5();e13722 [Pubmed: 26949251]

Quantitative Score

  • 289.6 [Mascot Score]

Throughput

  • High Throughput

Additional Notes

  • interactors identified using mass spectrometry, retaining hits for which either an untagged immunoprecipitation (IP) or IP of non-specific protein (TRIM39) produced fewer peptides hits and/or lower Mascot Scores

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLC25A6 MKI67
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3745129

Curated By

  • BioGRID