BAIT

KLK2

KLK2A2, hGK-1, hK2
kallikrein-related peptidase 2
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SERPINA3

AACT, ACT, GIG25, GIG24
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Alzheimer's Disease Project
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Human glandular kallikrein, hK2, shows arginine-restricted specificity and forms complexes with plasma protease inhibitors.

Mikolajczyk SD, Millar LS, Kumar A, Saedi MS

BACKGROUND: Human glandular kallikrein (hK2) is a new potential marker for prostate cancer. It is a serine protease expressed in human prostate epithelial cells which has 78% sequence identity with prostate-specific antigen (PSA). PSA is a widely used biochemical marker for prostate cancer. METHODS: Recombinant hK2 expressed in mammalian cells was purified to homogeneity by immunoaffinity chromatography, using an anti-hK2 ... [more]

Prostate Jan. 01, 1998; 34(1);44-50 [Pubmed: 9428387]

Throughput

  • Low Throughput

Curated By

  • BioGRID