GPR143
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA, IMP]
- calcium-mediated signaling using intracellular calcium source [IDA]
- eye pigment biosynthetic process [TAS]
- melanosome localization [IDA, ISS]
- melanosome organization [IMP]
- melanosome transport [IDA]
- neuropeptide signaling pathway [IDA]
- phosphatidylinositol-mediated signaling [IDA]
- regulation of calcium-mediated signaling [IDA]
- signal transduction [TAS]
- visual perception [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAI1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS]
- adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IBA]
- blood coagulation [TAS]
- cell division [IMP]
- platelet activation [TAS]
- response to peptide hormone [ISS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Ocular albinism: evidence for a defect in an intracellular signal transduction system.
G protein-coupled receptors (GPCRs) participate in the most common signal transduction system at the plasma membrane. The wide distribution of heterotrimeric G proteins in the internal membranes suggests that a similar signalling mechanism might also be used at intracellular locations. We provide here structural evidence that the protein product of the ocular albinism type 1 gene (OA1), a pigment cell-specific ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GPR143 GNAI1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID