BAIT
FASN
FAS, OA-519, SDR27X1
fatty acid synthase
GO Process (11)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- fatty acid metabolic process [TAS]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- osteoblast differentiation [IDA]
- pantothenate metabolic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NR5A2
B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2
nuclear receptor subfamily 5, group A, member 2
GO Process (9)
GO Function (8)
GO Component (3)
Gene Ontology Biological Process
- embryo development [TAS]
- endocrine pancreas development [TAS]
- gene expression [TAS]
- homeostatic process [NAS]
- intracellular receptor signaling pathway [TAS]
- positive regulation of transcription, DNA-templated [IDA, TAS]
- positive regulation of viral genome replication [IDA]
- regulation of transcription, DNA-templated [IDA, TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [TAS]
- phospholipid binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [TAS]
- phospholipid binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
Homo sapiens
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic positive genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID