Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.

Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, Rahman M, Brown KR, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid A, Pawling J, Lin ZY, Abdouni H, Wong CJ, Weiss A, Mero P, Dennis JW, Gingras AC, Myers CL, Andrews BJ, Boone C, Moffat J

The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]

Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)
phenotype: viability (PATO:0000169)

Additional Notes

CRISPR GI screen
Cell Line: HAP1
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: TKOv3
Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5

Curated By

BioGRID

Interaction Summary

FASN

Gene Ontology Biological Process

Gene Ontology Molecular Function

fatty acid synthase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

KDM5A

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]transcription coactivator activity [ISS]