BAIT
LDLR
FH, FHC, LDLCQ2
low density lipoprotein receptor
GO Process (15)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- cholesterol homeostasis [IMP]
- cholesterol import [ISS]
- cholesterol transport [IMP]
- endocytosis [TAS]
- intestinal cholesterol absorption [IMP]
- lipid metabolic process [TAS]
- lipoprotein metabolic process [TAS]
- low-density lipoprotein particle clearance [IMP]
- phospholipid transport [ISS]
- phototransduction, visible light [TAS]
- positive regulation of triglyceride biosynthetic process [ISS]
- receptor-mediated endocytosis [TAS]
- regulation of phosphatidylcholine catabolic process [ISS]
- retinoid metabolic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cell surface [IDA]
- clathrin-coated endocytic vesicle membrane [TAS]
- coated pit [IDA]
- early endosome [IDA]
- endosome membrane [TAS]
- external side of plasma membrane [IDA]
- integral component of plasma membrane [TAS]
- late endosome [IDA]
- lysosome [IDA]
- membrane [IDA]
- plasma membrane [TAS]
- receptor complex [IDA]
Homo sapiens
PREY
AGO2
EIF2C2, Q10
argonaute RISC catalytic component 2
GO Process (18)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Notch signaling pathway [TAS]
- RNA phosphodiester bond hydrolysis, endonucleolytic [EXP, IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- innate immune response [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translation involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translational initiation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISS]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISS]
- pre-miRNA processing [IDA]
- translation [NAS]
- translational initiation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic positive genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID