Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.

Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, Rahman M, Brown KR, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid A, Pawling J, Lin ZY, Abdouni H, Wong CJ, Weiss A, Mero P, Dennis JW, Gingras AC, Myers CL, Andrews BJ, Boone C, Moffat J

The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]

Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)
phenotype: viability (PATO:0000169)

Additional Notes

CRISPR GI screen
Cell Line: HAP1
Experimental Setup: Timecourse
GIST: A-phenotypic positive genetic interaction
Library: TKOv3
Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5

Curated By

BioGRID

Interaction Summary

LDLR

Gene Ontology Biological Process

Gene Ontology Molecular Function

clathrin heavy chain binding [TAS]glycoprotein binding [IPI]low-density lipoprotein receptor activity [IDA]protein binding [IPI]very-low-density lipoprotein particle receptor activity [IDA]

Gene Ontology Cellular Component

WDR48