Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

All Tcf HMG box transcription factors interact with Groucho-related co-repressors.

Brantjes H, Roose J, van De Wetering M, Clevers H

Tcf/Lef family transcription factors are the downstream effectors of the Wingless/Wnt signal transduction pathway. Upon Wingless/Wnt signalling, beta-catenin translocates to the nucleus, interacts with Tcf (1-3) and thus activates transcription of target genes (4,5). Tcf factors also interact with members of the Groucho (Grg/TLE) family of transcriptional co-repressors (6). We have now tested all known mammalian Groucho family members for ... [more]

Nucleic Acids Res. Apr. 01, 2001; 29(7);1410-9 [Pubmed: 11266540]

Throughput

  • Low Throughput

Curated By

  • BioGRID