BAIT
SREBF2
SREBP-2, SREBP2, bHLHd2, CTA-250D10.14-005
sterol regulatory element binding transcription factor 2
GO Process (10)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- cellular response to laminar fluid shear stress [NAS]
- lipid metabolic process [TAS]
- negative regulation of cholesterol efflux [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of cholesterol storage [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter [IDA]
- response to low-density lipoprotein particle [IEP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- C-8 sterol isomerase activity [IDA]
- E-box binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- C-8 sterol isomerase activity [IDA]
- E-box binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
PREY
PRDX5
ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, prx-V, SBBI10
peroxiredoxin 5
GO Process (12)
GO Function (8)
GO Component (12)
Gene Ontology Biological Process
- NADPH oxidation [IDA]
- cellular response to reactive oxygen species [IMP]
- hydrogen peroxide catabolic process [IDA]
- inflammatory response [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of oxidoreductase activity [IDA]
- negative regulation of transcription from RNA polymerase III promoter [IDA]
- positive regulation of collagen biosynthetic process [IDA]
- reactive nitrogen species metabolic process [IDA]
- regulation of apoptosis involved in tissue homeostasis [IDA]
- response to oxidative stress [IDA]
Gene Ontology Molecular Function- RNA polymerase III regulatory region DNA binding [IDA]
- antioxidant activity [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]
- peroxidase activity [IDA]
- peroxiredoxin activity [IDA]
- peroxynitrite reductase activity [IDA]
- protein dimerization activity [IDA]
- receptor binding [IPI]
- RNA polymerase III regulatory region DNA binding [IDA]
- antioxidant activity [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]
- peroxidase activity [IDA]
- peroxiredoxin activity [IDA]
- peroxynitrite reductase activity [IDA]
- protein dimerization activity [IDA]
- receptor binding [IPI]
Gene Ontology Cellular Component
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [IDA]
- mitochondrial matrix [TAS]
- mitochondrion [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- peroxisomal matrix [IDA]
- peroxisome [IDA]
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID