An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Transcription factor networks in Drosophila melanogaster.

Rhee DY, Cho DY, Zhai B, Slattery M, Ma L, Mintseris J, Wong CY, White KP, Celniker SE, Przytycka TM, Gygi SP, Obar RA, Artavanis-Tsakonas S

Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately ... [more]

Cell Rep Sep. 25, 2014; 8(6);2031-43 [Pubmed: 25242320]

Quantitative Score

62.506036 [HGSCore]

Throughput

High Throughput

Additional Notes

High confidence interactions were identified using a HyperGeometric Spectral Counts scoring method (HGSCore) and were filtered to a false discovery rate (FDR) of 2%.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DIP1 CG18011	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Giot L (2003)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CG18011

DIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded DNA binding [ISS]

Gene Ontology Cellular Component

Affinity Capture-MS