BAIT

TFIIEALPHA

CG10415, Dmel\CG10415, TFIIE, TFIIE-L, TfIIEa, dTFIIE-L, dTFIIEL, Dmel_CG10415
Transcription factor IIEalpha
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

TFIIEBETA

CG1276, Dmel\CG1276, E[[S]], TFIIE, TFIIbeta, dTFIIE-S, dTFIIES, Dmel_CG1276
Transcription factor IIEbeta
Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Transcription factor networks in Drosophila melanogaster.

Rhee DY, Cho DY, Zhai B, Slattery M, Ma L, Mintseris J, Wong CY, White KP, Celniker SE, Przytycka TM, Gygi SP, Obar RA, Artavanis-Tsakonas S

Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately ... [more]

Cell Rep Sep. 25, 2014; 8(6);2031-43 [Pubmed: 25242320]

Quantitative Score

  • 37.831623 [HGSCore]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified using a HyperGeometric Spectral Counts scoring method (HGSCore) and were filtered to a false discovery rate (FDR) of 2%.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TFIIEALPHA TFIIEBETA
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
TFIIEALPHA TFIIEBETA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
TFIIEALPHA TFIIEBETA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
3688104

Curated By

  • BioGRID