BAIT

CG18619

BcDNA:RH73910, Dmel\CG18619, Dmel_CG18619
CG18619 gene product from transcript CG18619-RD
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Drosophila melanogaster
PREY

CG13624

Dmel\CG13624, anon-WO0153538.65, Dmel_CG13624
CG13624 gene product from transcript CG13624-RB
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Transcription factor networks in Drosophila melanogaster.

Rhee DY, Cho DY, Zhai B, Slattery M, Ma L, Mintseris J, Wong CY, White KP, Celniker SE, Przytycka TM, Gygi SP, Obar RA, Artavanis-Tsakonas S

Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately ... [more]

Cell Rep Sep. 25, 2014; 8(6);2031-43 [Pubmed: 25242320]

Quantitative Score

  • 20.103109 [HGSCore]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified using a HyperGeometric Spectral Counts scoring method (HGSCore) and were filtered to a false discovery rate (FDR) of 2%.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CG13624 CG18619
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-FlyBase
-
CG13624 CG18619
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-FlyBase
-
CG18619 CG13624
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-FlyBase
-
CG13624 CG18619
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID