FRAT1
DVL1
Gene Ontology Biological Process
- Wnt signaling pathway, planar cell polarity pathway [IDA]
- canonical Wnt signaling pathway [IDA]
- dendrite morphogenesis [ISS]
- heart development [NAS]
- negative regulation of protein binding [IDA]
- negative regulation of protein kinase activity [IDA]
- neural tube development [IEP]
- neuromuscular junction development [ISS]
- neurotransmitter secretion [ISS]
- planar cell polarity pathway involved in neural tube closure [IBA]
- positive regulation of Wnt signaling pathway [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein localization to nucleus [IMP]
- receptor clustering [ISS]
- regulation of neurotransmitter levels [ISS]
- synapse organization [ISS]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Axin and Frat1 interact with dvl and GSK, bridging Dvl to GSK in Wnt-mediated regulation of LEF-1.
Wnt proteins transduce their signals through dishevelled (Dvl) proteins to inhibit glycogen synthase kinase 3beta (GSK), leading to the accumulation of cytosolic beta-catenin and activation of TCF/LEF-1 transcription factors. To understand the mechanism by which Dvl acts through GSK to regulate LEF-1, we investigated the roles of Axin and Frat1 in Wnt-mediated activation of LEF-1 in mammalian cells. We found ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID