An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses.

Izawa I, Nishizawa M, Ohtakara K, Inagaki M

Densin-180, a protein purified from the postsynaptic density fraction of the rat forebrain, is the founding member of a newly described family of proteins termed the LAP (leucine-rich repeats and PSD-95/Dlg-A/ZO-1 (PDZ) domains) family that plays essential roles in establishment of cell polarity. To identify Densin-180-binding proteins, we screened a yeast two-hybrid library using the carboxyl-terminal fragment of Densin-180 containing ... [more]

J. Biol. Chem. Feb. 15, 2002; 277(7);5345-50 [Pubmed: 11729199]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LRRC7 CTNND2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Izawa I (2002)	Low	-	BioGRID	-
LRRC7 CTNND2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Izawa I (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

LRRC7

Gene Ontology Molecular Function

protein binding [IPI]

CTNND2

Gene Ontology Biological Process