MAPK8
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- JNK cascade [IDA, TAS]
- JUN phosphorylation [IDA]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- cellular response to lipopolysaccharide [IDA]
- cellular response to mechanical stimulus [IEP]
- innate immune response [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of protein binding [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA, IMP]
- positive regulation of apoptotic process [TAS]
- positive regulation of deacetylase activity [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of histone deacetylation [IMP]
- regulation of protein localization [IDA]
- regulation of sequence-specific DNA binding transcription factor activity [TAS]
- response to UV [IDA]
- response to stress [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
IRS1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- cellular response to insulin stimulus [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glucose homeostasis [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IDA, IMP, IPI, TAS]
- insulin-like growth factor receptor signaling pathway [IPI]
- negative regulation of insulin receptor signaling pathway [ISS]
- negative regulation of insulin secretion [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [NAS]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose import in response to insulin stimulus [IDA]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of insulin receptor signaling pathway [ISS]
- positive regulation of phosphatidylinositol 3-kinase activity [ISS]
- response to insulin [IDA]
- response to peptide hormone [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The c-Jun NH(2)-terminal kinase promotes insulin resistance during association with insulin receptor substrate-1 and phosphorylation of Ser(307).
Tumor necrosis factor alpha (TNFalpha) inhibits insulin action, in part, through serine phosphorylation of IRS proteins; however, the phosphorylation sites that mediate the inhibition are unknown. TNFalpha promotes multipotential signal transduction cascades, including the activation of the Jun NH(2)-terminal kinase (JNK). Endogenous JNK associates with IRS-1 in Chinese hamster ovary cells. Anisomycin, a strong activator of JNK in these cells, ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
MAPK8 IRS1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
MAPK8 IRS1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID