BAIT
MBK-2
CELE_F49E11.1
Protein MBK-2
GO Process (19)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- asymmetric protein localization involved in cell fate determination [IMP]
- body morphogenesis [IMP]
- cytokinesis [IMP]
- embryo development ending in birth or egg hatching [IMP]
- embryonic pattern specification [IMP]
- meiotic chromosome segregation [IMP]
- meiotic nuclear division [IMP]
- microtubule-based process [IMP]
- mitotic cytokinesis [IMP]
- mitotic spindle organization [IMP]
- peptidyl-serine phosphorylation [ISS]
- peptidyl-threonine phosphorylation [ISS]
- peptidyl-tyrosine phosphorylation [ISS]
- positive regulation of protein catabolic process [IMP]
- pronuclear migration [IMP]
- protein autophosphorylation [ISS]
- protein phosphorylation [IDA]
- reproduction [IMP]
- vulval development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
CLS-2
CELE_R107.6
Protein CLS-2
GO Process (8)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics.
Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early ... [more]
Unknown Dec. 01, 2015; 15(5);1642-57 [Pubmed: 26912668]
Throughput
- High Throughput
Additional Notes
- an interaction was considered high confidence using a measure that combined fold-change of prey abundance as well as p-value of a Student's t-test for experimental versus control purifications
- the bait protein was affinity purified and the prey protein was identified as an interactor by mass spectrometry (MS)
- the p-value is reported here
Curated By
- BioGRID