BAIT

IL1R2

CD121b, Il1r-2
interleukin 1 receptor, type II
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY

IL1RAP

C3orf13, IL-1RAcP, IL1R3
interleukin 1 receptor accessory protein
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

The type II IL-1 receptor interacts with the IL-1 receptor accessory protein: a novel mechanism of regulation of IL-1 responsiveness.

Lang D, Knop J, Wesche H, Raffetseder U, Kurrle R, Boraschi D, Martin MU

IL-1 binds to two types of receptors on the cell membrane, of which only type I (IL-1RI) transduces signals in concert with the coreceptor IL-1 receptor accessory protein (IL-1RAcP) while type II (IL-1RII) allegedly functions solely as ligand sink and decoy receptor without participating in IL-1 signaling. To investigate the regulatory role of IL-1RII on IL-1 responsiveness, a chimeric receptor ... [more]

J. Immunol. Dec. 15, 1998; 161(12);6871-7 [Pubmed: 9862719]

Throughput

  • Low Throughput

Additional Notes

  • Radiolabeled ligand

Curated By

  • BioGRID