BAIT

P53

CG10873, CG31325, CG33336, D-p53, DMP53, Dm-P53, Dmel\CG33336, Dp53, prac, Dmel_CG33336
CG33336 gene product from transcript CG33336-RB
GO Process (38)
GO Function (12)
GO Component (5)

Gene Ontology Biological Process

Drosophila melanogaster
PREY

BIP2

CG2009, DmTAF3, Dmel\CG2009, TAF155, TAF3, TAFII155, TAF[[II]]155, TFIID, bip-II, dBIP2, dTAFII3, dTAF[[II]]155, i163, i31, Dmel_CG2009
CG2009 gene product from transcript CG2009-RA
Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.

Bereczki O, Ujfaludi Z, Pardi N, Nagy Z, Tora L, Boros IM, Balint E

BACKGROUND: The tumour suppressor protein p53 is a sequence specific DNA-binding transcription regulator, which exerts its versatile roles in genome protection and apoptosis by affecting the expression of a large number of genes. In an attempt to obtain a better understanding of the mechanisms by which p53 transcription function is regulated, we studied p53 interactions. RESULTS: We identified BIP2 (Bric-a-brac ... [more]

BMC Mol. Biol. Jun. 14, 2008; 9(0);57 [Pubmed: 18549481]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
P53 BIP2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-FlyBase
-
P53 BIP2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
443576

Curated By