INSR
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- activation of MAPK activity [IMP]
- activation of protein kinase B activity [IDA]
- activation of protein kinase activity [IMP]
- cellular response to insulin stimulus [IDA]
- glucose homeostasis [IMP]
- heart morphogenesis [IMP]
- insulin receptor signaling pathway [IDA, TAS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of DNA replication [IMP]
- positive regulation of MAPK cascade [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IC, IDA]
- positive regulation of developmental growth [IMP]
- positive regulation of glucose import [IDA, NAS]
- positive regulation of glycogen biosynthetic process [IDA]
- positive regulation of glycolytic process [IMP]
- positive regulation of mitosis [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of respiratory burst [IDA]
- protein autophosphorylation [IDA, IMP]
- protein heterotetramerization [IDA]
- regulation of embryonic development [IMP]
- regulation of transcription, DNA-templated [IMP]
- signal transduction by phosphorylation [IDA]
- transformation of host cell by virus [IMP]
Gene Ontology Molecular Function- ATP binding [IDA]
- GTP binding [IDA]
- PTB domain binding [IPI]
- insulin binding [IDA, IPI]
- insulin receptor substrate binding [IPI]
- insulin-activated receptor activity [IDA]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor II binding [IPI]
- insulin-like growth factor receptor binding [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, IMP]
- receptor signaling protein tyrosine kinase activity [IDA]
- ATP binding [IDA]
- GTP binding [IDA]
- PTB domain binding [IPI]
- insulin binding [IDA, IPI]
- insulin receptor substrate binding [IPI]
- insulin-activated receptor activity [IDA]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor II binding [IPI]
- insulin-like growth factor receptor binding [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, IMP]
- receptor signaling protein tyrosine kinase activity [IDA]
Gene Ontology Cellular Component
GRB2
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- Ras protein signal transduction [TAS]
- T cell costimulation [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell-cell signaling [TAS]
- cellular response to ionizing radiation [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IPI, TAS]
- leukocyte migration [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- platelet activation [TAS]
- positive regulation of reactive oxygen species metabolic process [IMP]
- receptor internalization [IMP]
- signal transduction in response to DNA damage [IMP]
Gene Ontology Molecular Function- SH3 domain binding [IDA]
- SH3/SH2 adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [IPI]
- identical protein binding [IPI]
- insulin receptor substrate binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- SH3 domain binding [IDA]
- SH3/SH2 adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [IPI]
- identical protein binding [IPI]
- insulin receptor substrate binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Functional importance of Shc tyrosine 317 on insulin signaling in Rat1 fibroblasts expressing insulin receptors.
Shc is phosphorylated on Tyr-317, which serves as a docking site for Grb2. To investigate the specific role of Shc phosphorylation and Shc.Grb2 coupling on insulin signaling, we generated expression vectors for wild-type (WT-Shc) and a mutant Shc with a Tyr-317 --> Phe substitution (317Y/F-Shc) and stably transfected them into Rat1 fibroblasts expressing insulin receptors (HIRc). From different clonal cell ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INSR GRB2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 0 | BioGRID | 3509459 |
Curated By
- BioGRID