BAIT

PTK2

FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK
protein tyrosine kinase 2
GO Process (42)
GO Function (8)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens
PREY

EPHA2

ARCC2, CTPA, CTPP1, CTRCT6, ECK
EPH receptor A2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Activation of EphA2 kinase suppresses integrin function and causes focal-adhesion-kinase dephosphorylation.

Miao H, Burnett E, Kinch M, Simon E, Wang B

Interactions between receptor tyrosine kinases of the Eph family and their ligands, ephrins, are implicated in establishment of organ boundaries and repulsive guidance of cell migration during development, but the mechanisms by which this is achieved are unclear. Here we show that activation of endogenous EphA2 kinase induces an inactive conformation of integrins and inhibits cell spreading, migration and integrin-mediated ... [more]

Nat. Cell Biol. Feb. 01, 2000; 2(2);62-9 [Pubmed: 10655584]

Throughput

  • Low Throughput

Curated By

  • BioGRID