Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Functional and physical interactions of the adaptor protein complex AP-4 with ADP-ribosylation factors (ARFs).

Boehm M, Aguilar RC, Bonifacino JS

AP-4 is a member of the family of heterotetrameric adaptor protein (AP) complexes that mediate the sorting of integral membrane proteins in post-Golgi compartments. This complex consists of four subunits (epsilon, beta4, mu4 and sigma4) and localizes to the cytoplasmic face of the trans-Golgi network (TGN). Here, we show that the recruitment of endogenous AP-4 to the TGN in vivo ... [more]

EMBO J. Nov. 15, 2001; 20(22);6265-76 [Pubmed: 11707398]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ARF1 AP4M1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Li FL (2022)	High	-	BioGRID	3411818

Curated By

BioGRID

Interaction Summary

ARF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]receptor signaling protein activity [TAS]

Gene Ontology Cellular Component

AP4M1