An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Isolation of a 40-kDa Huntingtin-associated protein.

Peters MF, Ross CA

Huntington's disease is caused by an expanded CAG trinucleotide repeat coding for a polyglutamine stretch within the huntingtin protein. Currently, the function of normal huntingtin and the mechanism by which expanded huntingtin causes selective neurotoxicity remain unknown. Clues may come from the identification of huntingtin-associated proteins (HAPs). Here, we show that huntingtin copurifies with a single novel 40-kDa protein termed ... [more]

J. Biol. Chem. Feb. 02, 2001; 276(5);3188-94 [Pubmed: 11035034]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
F8A1 HTT	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3096153

Curated By

BioGRID

Interaction Summary

HTT

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-tubulin binding [IDA]dynactin binding [IPI]dynein intermediate chain binding [IDA]identical protein binding [IPI]ion channel binding [IDA]p53 binding [IPI]protein binding [IPI]transcription factor binding [IBA]

Gene Ontology Cellular Component

F8A1