PHYL
Gene Ontology Biological Process
- R1/R6 cell fate commitment [IEP, IMP]
- R7 cell development [TAS]
- R7 cell fate commitment [IEP, IMP]
- Ras protein signal transduction [IGI]
- compound eye photoreceptor cell differentiation [IMP]
- lateral inhibition [IMP]
- peripheral nervous system development [IMP]
- proteasomal protein catabolic process [IDA]
- regulation of Notch signaling pathway [IDA]
- regulation of Wnt signaling pathway [IDA]
- sensory organ boundary specification [IMP]
- sensory organ precursor cell fate determination [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SINAH
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Two modes of degradation of the tramtrack transcription factors by Siah homologues.
The Siah proteins, mammalian homologues of the Drosophila Sina protein, function as ubiquitin-protein isopeptide ligase enzymes to target a wide range of cellular proteins for degradation. We report here a novel Drosophila protein that is homologous to Sina, named Sina-Homologue (SinaH). We show that it can direct the degradation of the transcriptional repressor Tramtrack (Ttk) using two different mechanisms. One ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PHYL SINAH | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |