An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain.

Ye H, Ojelade SA, Li-Kroeger D, Zuo Z, Wang L, Li Y, Gu JY, Tepass U, Rodal AA, Bellen HJ, Shulman JM

Retromer, including Vps35, Vps26, and Vps29, is a protein complex responsible for recycling proteins within the endolysosomal pathway. Although implicated in both Parkinson's and Alzheimer's disease, our understanding of retromer function in the adult brain remains limited, in part because Vps35 and Vps26 are essential for development. In Drosophila, we find that Vps29 is dispensable for embryogenesis but required for ... [more]

Elife Apr. 14, 2020; 9(); [Pubmed: 32286230]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VPS29 VPS35	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Giot L (2003)	High	-	BioGRID	-

Curated By

FlyBase

Interaction Summary

VPS35

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IPI]protein transporter activity [ISS]

Gene Ontology Cellular Component

VPS29