PREY

NFKB2

CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p52
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Characterization of mechanisms involved in transrepression of NF-kappa B by activated glucocorticoid receptors.

Scheinman RI, Gualberto A, Jewell CM, Cidlowski JA, Baldwin AS

Glucocorticoids are potent immunosuppressants which work in part by inhibiting cytokine gene transcription. We show here that NF-kappa B, an important regulator of numerous cytokine genes, is functionally inhibited by the synthetic glucocorticoid dexamethasone (DEX). In transfection experiments, DEX treatment in the presence of cotransfected glucocorticoid receptor (GR) inhibits NF-kappa B p65-mediated gene expression and p65 inhibits GR activation of ... [more]

Mol. Cell. Biol. Feb. 01, 1995; 15(2);943-53 [Pubmed: 7823959]

Throughput

  • Low Throughput

Curated By

  • BioGRID