PREY

HCK

JTK9, p59Hck, p61Hck, RP5-836N17.3
HCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (4)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Novel association of the src family kinases, hck and c-fgr, with CCR3 receptor stimulation: A possible mechanism for eotaxin-induced human eosinophil chemotaxis.

El-Shazly A, Yamaguchi N, Masuyama K, Suda T, Ishikawa T

The chemokine eotaxin is a potent and relatively eosinophil-specific chemoattractant implicated in the cell migration to inflammatory sites in allergic diseases. Eotaxin exerts its activity solely through the CCR3 receptor, but the signaling pathways are poorly defined. In this study, we show that eotaxin induces an increase in tyrosine phosphorylation of multiple cellular proteins in normal human eosinophils. Eotaxin-dependent tyrosine ... [more]

Biochem. Biophys. Res. Commun. Oct. 14, 1999; 264(1);163-70 [Pubmed: 10527858]

Throughput

  • Low Throughput

Curated By

  • BioGRID