CAF1
Gene Ontology Biological Process
- DNA repair [TAS]
- DNA replication-dependent nucleosome assembly [NAS]
- chromatin assembly [IDA, ISS]
- chromatin silencing [IPI]
- dendrite morphogenesis [IMP]
- eggshell chorion gene amplification [IC]
- histone acetylation [IDA, ISS]
- histone methylation [IDA]
- mitotic cytokinesis [IMP]
- muscle organ development [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- neuron development [IMP]
- neuron projection morphogenesis [IMP]
- nucleosome assembly [IDA, NAS]
- nucleosome mobilization [IDA]
- nucleosome positioning [IDA]
- positive regulation of cell proliferation [IMP]
- regulation of histone H3-K27 methylation [IMP]
- regulation of mitotic cell cycle [IMP]
- segment specification [IMP]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- chromatin binding [NAS]
- histone acetyltransferase binding [IPI, ISS]
- histone binding [IDA, NAS, TAS]
- histone deacetylase binding [IDA, ISS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K27 specific) [IC]
- histone methyltransferase activity (H3-K9 specific) [IC]
- nucleosome binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- chromatin binding [NAS]
- histone acetyltransferase binding [IPI, ISS]
- histone binding [IDA, NAS, TAS]
- histone deacetylase binding [IDA, ISS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K27 specific) [IC]
- histone methyltransferase activity (H3-K9 specific) [IC]
- nucleosome binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
Gene Ontology Cellular Component
CAF1-180
Gene Ontology Biological Process
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Localization of Drosophila CENP-A to non-centromeric sites depends on the NuRD complex.
Centromere function requires the presence of the histone H3 variant CENP-A in most eukaryotes. The precise localization and protein amount of CENP-A are crucial for correct chromosome segregation, and misregulation can lead to aneuploidy. To characterize the loading of CENP-A to non-centromeric chromatin, we utilized different truncation- and localization-deficient CENP-A mutant constructs in Drosophila melanogaster cultured cells, and show that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CAF1 CAF1-180 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| CAF1 CAF1-180 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CAF1-180 CAF1 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - |