Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor.

Lee JW, Choi HS, Gyuris J, Brent R, Moore DD

The thyroid hormone (T3) receptors (TRs) are hormone-dependent transcription factors that regulate expression of a variety of specific target genes. To help elucidate the mechanisms that underlie this transcriptional regulation and other potential TR activities, we used the yeast interaction trap to isolate clones encoding proteins that specifically interact with the ligand binding domain of the rat TR beta. Several ... [more]

Mol. Endocrinol. Feb. 01, 1995; 9(2);243-54 [Pubmed: 7776974]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

THRB

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin DNA binding [ISO]double-stranded DNA binding [IDA]enzyme binding [ISO]ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [TAS]protein homodimerization activity [IDA]thyroid hormone binding [ISO, ISS]thyroid hormone receptor activity [IDA, ISO]

Gene Ontology Cellular Component

HMGN3