BIP2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TRL
Gene Ontology Biological Process
- cellular process [IMP]
- chromatin assembly [IDA]
- chromatin organization [NAS]
- dosage compensation [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- negative regulation of transcription, DNA-templated [IGI]
- nuclear division [IMP]
- oogenesis [IMP]
- positive regulation of chromatin silencing [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP, TAS]
- protein oligomerization [IDA]
- regulation of transcription from RNA polymerase II promoter [NAS]
- regulation of transcription, DNA-templated [NAS]
- spermatogenesis [IMP]
- syncytial blastoderm mitotic cell cycle [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Transcriptional activation by GAGA factor is through its direct interaction with dmTAF3.
The GAGA factor (GAF), encoded by the Trithorax like gene (Trl) is a multifunctional protein involved in gene activation, Polycomb-dependent repression, chromatin remodeling and is a component of chromatin domain boundaries. Although first isolated as transcriptional activator of the Drosophila homeotic gene Ultrabithorax (Ubx), the molecular basis of this GAF activity is unknown. Here we show that dmTAF3 (also known ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRL BIP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| TRL BIP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| BIP2 TRL | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |