BAIT
SRF
MCM1
serum response factor (c-fos serum response element-binding transcription factor)
GO Process (23)
GO Function (9)
GO Component (2)
Gene Ontology Biological Process
- angiogenesis involved in wound healing [TAS]
- cell migration involved in sprouting angiogenesis [IMP]
- cellular senescence [IMP]
- heart development [ISS]
- heart looping [ISS]
- mRNA transcription from RNA polymerase II promoter [ISS]
- muscle cell cellular homeostasis [ISS]
- negative regulation of beta-amyloid clearance [IMP]
- neuron development [TAS]
- positive regulation of cell differentiation [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of smooth muscle contraction [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [IGI]
- positive regulation of transcription initiation from RNA polymerase II promoter [IDA]
- positive regulation of transcription via serum response element binding [IDA]
- regulation of smooth muscle cell differentiation [TAS]
- response to cytokine [IMP, NAS]
- response to hormone [IDA]
- response to hypoxia [IEP]
- response to toxic substance [TAS]
- transcription from RNA polymerase II promoter [IDA]
- trophectodermal cell differentiation [IDA]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- serum response element binding [IDA]
- transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- serum response element binding [IDA]
- transcription factor binding [IPI]
Homo sapiens
PREY
NKX3-1
BAPX2, NKX3, NKX3.1, NKX3A
NK3 homeobox 1
GO Process (40)
GO Function (14)
GO Component (2)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- androgen receptor signaling pathway [IDA]
- branching involved in prostate gland morphogenesis [ISS]
- branching morphogenesis of an epithelial tube [ISS]
- cellular response to drug [IEP]
- cellular response to hypoxia [IDA]
- cellular response to interleukin-1 [IEP]
- cellular response to steroid hormone stimulus [IDA]
- cellular response to tumor necrosis factor [IEP]
- epithelial cell proliferation involved in salivary gland morphogenesis [ISS]
- mitotic cell cycle arrest [IDA]
- multicellular organismal development [TAS]
- negative regulation of cell proliferation [IDA]
- negative regulation of epithelial cell proliferation [ISS]
- negative regulation of epithelial cell proliferation involved in prostate gland development [ISS]
- negative regulation of estrogen receptor binding [IDA]
- negative regulation of gene expression [IDA]
- negative regulation of insulin-like growth factor receptor signaling pathway [ISS]
- negative regulation of mitotic cell cycle [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of androgen secretion [IDA]
- positive regulation of apoptotic signaling pathway [IDA]
- positive regulation of cell death [IDA]
- positive regulation of cell division [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of gene expression [IDA, IMP]
- positive regulation of intrinsic apoptotic signaling pathway [IDA]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of response to DNA damage stimulus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IMP, ISS]
- protein kinase B signaling [IMP]
- regulation of transcription, DNA-templated [NAS]
- response to testosterone [ISS]
- salivary gland development [ISS]
- steroid hormone mediated signaling pathway [IDA]
Gene Ontology Molecular Function- androgen receptor activity [IDA]
- core promoter binding [IDA]
- cysteine-type endopeptidase activator activity involved in apoptotic process [IDA]
- estrogen receptor activity [IDA]
- estrogen receptor binding [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase activator activity [IDA]
- protein self-association [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transcription regulatory region sequence-specific DNA binding [IDA]
- androgen receptor activity [IDA]
- core promoter binding [IDA]
- cysteine-type endopeptidase activator activity involved in apoptotic process [IDA]
- estrogen receptor activity [IDA]
- estrogen receptor binding [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase activator activity [IDA]
- protein self-association [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transcription regulatory region sequence-specific DNA binding [IDA]
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The smooth muscle gamma-actin gene promoter is a molecular target for the mouse bagpipe homologue, mNkx3-1, and serum response factor.
An evolutionarily conserved vertebrate homologue of the Drosophila NK-3 homeodomain gene bagpipe, Nkx3-1, is expressed in vascular and visceral mesoderm-derived muscle tissues and may influence smooth muscle cell differentiation. Nkx3-1 was evaluated for mediating smooth muscle gamma-actin (SMGA) gene activity, a specific marker of smooth muscle differentiation. Expression of mNkx3-1 in heterologous CV-1 fibroblasts was unable to elicit SMGA promoter ... [more]
J. Biol. Chem. Dec. 15, 2000; 275(50);39061-72 [Pubmed: 10993896]
Throughput
- Low Throughput
Curated By
- BioGRID