BAIT

HNRNPH1

HNRPH, HNRPH1, hnRNPH
heterogeneous nuclear ribonucleoprotein H1 (H)
GO Process (5)
GO Function (4)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

ACTN4

ACTININ-4, FSGS, FSGS1
actinin, alpha 4
Human Papilloma Virus (HPV) Project
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

High-throughput analyses of hnRNP H1 dissects its multi-functional aspect.

Uren PJ, Bahrami-Samani E, de Araujo PR, Vogel C, Qiao M, Burns SC, Smith AD, Penalva LO

hnRNPs are polyvalent RNA binding proteins that have been implicated in a range of regulatory roles including splicing, mRNA decay, translation, and miRNA metabolism. A variety of genome wide studies have taken advantage of methods like CLIP and RIP to identify the targets and binding sites of RNA binding proteins. However, due to the complex nature of RNA-binding proteins, these ... [more]

RNA Biol Jan. 14, 2016; 13(4);400-11 [Pubmed: 26760575]

Throughput

  • High Throughput

Additional Notes

  • RIP-Seq
  • iCLIP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ACTN4 HNRNPH1
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3675980

Curated By

  • BioGRID