BAIT

HNRNPH1

HNRPH, HNRPH1, hnRNPH
heterogeneous nuclear ribonucleoprotein H1 (H)
GO Process (5)
GO Function (4)
GO Component (4)
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

High-throughput analyses of hnRNP H1 dissects its multi-functional aspect.

Uren PJ, Bahrami-Samani E, de Araujo PR, Vogel C, Qiao M, Burns SC, Smith AD, Penalva LO

hnRNPs are polyvalent RNA binding proteins that have been implicated in a range of regulatory roles including splicing, mRNA decay, translation, and miRNA metabolism. A variety of genome wide studies have taken advantage of methods like CLIP and RIP to identify the targets and binding sites of RNA binding proteins. However, due to the complex nature of RNA-binding proteins, these ... [more]

RNA Biol Jan. 14, 2016; 13(4);400-11 [Pubmed: 26760575]

Throughput

  • High Throughput

Additional Notes

  • RIP-Seq
  • iCLIP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ADARB1 HNRNPH1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3544084

Curated By

  • BioGRID