An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

High-throughput analyses of hnRNP H1 dissects its multi-functional aspect.

Uren PJ, Bahrami-Samani E, de Araujo PR, Vogel C, Qiao M, Burns SC, Smith AD, Penalva LO

hnRNPs are polyvalent RNA binding proteins that have been implicated in a range of regulatory roles including splicing, mRNA decay, translation, and miRNA metabolism. A variety of genome wide studies have taken advantage of methods like CLIP and RIP to identify the targets and binding sites of RNA binding proteins. However, due to the complex nature of RNA-binding proteins, these ... [more]

RNA Biol Jan. 14, 2016; 13(4);400-11 [Pubmed: 26760575]

Throughput

High Throughput

Additional Notes

RIP-Seq
iCLIP

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
POU2F1 HNRNPH1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Du L (2016)	High	6.75	BioGRID	2952659

Curated By

BioGRID

Interaction Summary

HNRNPH1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]poly(A) RNA binding [IDA]poly(U) RNA binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

POU2F1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding [IDA]