An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Heterochromatin formation in mammalian cells: interaction between histones and HP1 proteins.

Nielsen AL, Oulad-Abdelghani M, Ortiz JA, Remboutsika E, Chambon P, Losson R

Members of the heterochromatin protein 1 (HP1) family are silencing nonhistone proteins. Here, we show that in P19 embryonal carcinoma (EC) nuclei, HP1 alpha, beta, and gamma form homo- and heteromers associated with nucleosomal core histones. In vitro, all three HP1s bind to tailed and tailless nucleosomes and specifically interact with the histone-fold of histone H3. Furthermore, HP1alpha interacts with ... [more]

Mol. Cell Apr. 01, 2001; 7(4);729-39 [Pubmed: 11336697]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CBX5

Gene Ontology Biological Process

Gene Ontology Molecular Function

methylated histone binding [IDA]protein binding [IPI]protein binding, bridging [ISS]repressing transcription factor binding [ISS]

Gene Ontology Cellular Component

HIST3H2A