UBE2I
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of intracellular steroid hormone receptor signaling pathway [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein sumoylation [IBA, ISO]
- protein ubiquitination [IBA, ISO]
- regulation of receptor activity [ISO]
Gene Ontology Molecular Function- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
SLC2A1
Gene Ontology Biological Process
- L-ascorbic acid metabolic process [TAS]
- carbohydrate metabolic process [TAS]
- energy reserve metabolic process [TAS]
- glucose transport [IDA, TAS]
- hexose transport [TAS]
- protein complex assembly [IDA]
- regulation of insulin secretion [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
The sentrin-conjugating enzyme mUbc9 interacts with GLUT4 and GLUT1 glucose transporters and regulates transporter levels in skeletal muscle cells.
Glucose transport in insulin-regulated tissues is mediated by the GLUT4 and GLUT1 transporters. Using the yeast two-hybrid system, we have cloned the sentrin-conjugating enzyme mUbc9 as a protein that interacts with the GLUT4 COOH-terminal intracellular domain. The mUbc9 enzyme was found to bind directly to GLUT4 and GLUT1 through an 11-aa sequence common to the two transporters and to modify ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID