BAIT
ALG-1
CELE_F48F7.1
Protein ALG-1
GO Process (10)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- embryo development [IGI]
- embryo development ending in birth or egg hatching [IMP]
- germ cell development [IMP]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- molting cycle, collagen and cuticulin-based cuticle [IMP]
- nematode larval development [IGI, IMP]
- positive regulation of multicellular organism growth [IMP]
- reproduction [IMP]
- vulval development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
DPY-18
CELE_Y47D3B.10, phy-1, Y47D3B.10
dpy-18 encodes a catalytic alpha subunit of collagen prolyl 4-hydroxylase (P4H) orthologous to human P4H alpha (I) isoform (OMIM: 176710), an alpha subunit of prolyl-4-hydroxylase which is a procollagen modifying enzyme required for exoskeleton formation, morphogenesis and maintenance of body shape; dpy-18 has a critical role in hydroxylation of cuticle collagens during collagen synthesis; dpy-18, in complex with phy-2, is essential for the survival of the C. elegans; dpy-18 mutations alone result in a mild dumpy phenotype; expressed dpy-18 and ten-1 act in parallel influencing the connection between epidermis and muscle during embryonic development; deletion of dpy-18 causes aggregation of collagen IV in body wall muscles in elongated embryos and triggers the loss of tissue integrity in ten-1 mutants; dpy-18 is expressed throughout the hypodermis, head and posterior neurons.
GO Process (10)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- body morphogenesis [IMP]
- collagen and cuticulin-based cuticle development [IMP]
- embryo development [IMP]
- embryo development ending in birth or egg hatching [IMP]
- embryonic body morphogenesis [IGI]
- locomotion [IMP]
- macromolecule modification [IDA]
- oxidation-reduction process [IDA]
- peptidyl-proline hydroxylation [IDA]
- positive regulation of multicellular organism growth [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Caenorhabditis elegans ALG-1 antimorphic mutations uncover functions for Argonaute in microRNA guide strand selection and passenger strand disposal.
MicroRNAs are regulators of gene expression whose functions are critical for normal development and physiology. We have previously characterized mutations in a Caenorhabditis elegans microRNA-specific Argonaute ALG-1 (Argonaute-like gene) that are antimorphic [alg-1(anti)]. alg-1(anti) mutants have dramatically stronger microRNA-related phenotypes than animals with a complete loss of ALG-1. ALG-1(anti) miRISC (microRNA induced silencing complex) fails to undergo a functional transition ... [more]
Proc Natl Acad Sci U S A Sep. 22, 2015; 112(38);E5271-80 [Pubmed: 26351692]
Quantitative Score
- 22.53 [Normalized Spectral Abundance Factor]
Throughput
- High Throughput
Additional Notes
- the maximum background-corrected normalized spectral abundance factor (NSAF) obtained from purifications of any of the bait variants is reported here
- the prey protein was identified in at least one of purifications
- wild type and several mutant versions of Alg-1 were affinity purified and associated proteins were identified by mass spectrometry
Curated By
- BioGRID