PLN
Gene Ontology Biological Process
- blood circulation [NAS]
- cytosolic calcium ion homeostasis [IC]
- negative regulation of ATPase activity [IDA, ISS]
- negative regulation of calcium ion binding [IDA, ISS]
- negative regulation of calcium ion import [ISS]
- negative regulation of calcium ion import into sarcoplasmic reticulum [ISS]
- negative regulation of calcium ion transmembrane transporter activity [IDA, ISS]
- negative regulation of calcium ion transport [IDA]
- negative regulation of calcium-transporting ATPase activity [IDA]
- negative regulation of catalytic activity [ISS]
- negative regulation of heart rate [IMP]
- regulation of calcium ion transport [IDA, ISS]
- regulation of calcium-transporting ATPase activity [IDA]
- regulation of cardiac muscle cell contraction [IC]
- regulation of cardiac muscle cell membrane potential [IC]
- regulation of heart contraction [IMP]
- regulation of relaxation of cardiac muscle [IC]
- regulation of the force of heart contraction [IC]
- relaxation of cardiac muscle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ATP2A1
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- apoptotic mitochondrial changes [IMP]
- blood coagulation [TAS]
- calcium ion import [IMP]
- calcium ion transmembrane transport [IDA]
- calcium ion transport [IDA, IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- ion transmembrane transport [TAS]
- maintenance of mitochondrion location [IMP]
- negative regulation of endoplasmic reticulum calcium ion concentration [IMP]
- negative regulation of striated muscle contraction [IMP]
- positive regulation of endoplasmic reticulum calcium ion concentration [IMP]
- positive regulation of fast-twitch skeletal muscle fiber contraction [IDA]
- positive regulation of mitochondrial calcium ion concentration [IMP]
- regulation of striated muscle contraction [IMP]
- relaxation of skeletal muscle [IDA]
- response to endoplasmic reticulum stress [IMP]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- H zone [IDA]
- I band [IDA]
- calcium channel complex [IC]
- endoplasmic reticulum membrane [IDA, TAS]
- endoplasmic reticulum-Golgi intermediate compartment [ISS]
- integral component of membrane [NAS]
- membrane [ISS]
- perinuclear region of cytoplasm [ISS]
- platelet dense tubular network membrane [TAS]
- sarcoplasmic reticulum [ISS, NAS]
- sarcoplasmic reticulum membrane [IC, TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Phospholamban domain IB forms an interaction site with the loop between transmembrane helices M6 and M7 of sarco(endo)plasmic reticulum Ca2+ ATPases.
Transmembrane helix M6 of the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) has been shown to form a site of interaction with phospholamban (PLN). Site-directed mutagenesis was carried out in the cytoplasmic loop (L67) between M6 and M7 in SERCA1a to detect other SERCA-PLN binding sites. Mutants N810A, D813A, and R822A had diminished ability to interact functionally with PLN, but only D813A ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PLN ATP2A1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PLN ATP2A1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID