BAIT

ALG-1

CELE_F48F7.1
Protein ALG-1
Caenorhabditis elegans
PREY

COPB-1

Y25C1A.5, CELE_Y25C1A.5
Y25C1A.5 encodes a beta subunit of the coatomer (COPI) complex; in mass RNAi assays, Y25C1A.5 is required for fertility, adult viability, osmoregulation, and general health.
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Caenorhabditis elegans ALG-1 antimorphic mutations uncover functions for Argonaute in microRNA guide strand selection and passenger strand disposal.

Zinovyeva AY, Veksler-Lublinsky I, Vashisht AA, Wohlschlegel JA, Ambros VR

MicroRNAs are regulators of gene expression whose functions are critical for normal development and physiology. We have previously characterized mutations in a Caenorhabditis elegans microRNA-specific Argonaute ALG-1 (Argonaute-like gene) that are antimorphic [alg-1(anti)]. alg-1(anti) mutants have dramatically stronger microRNA-related phenotypes than animals with a complete loss of ALG-1. ALG-1(anti) miRISC (microRNA induced silencing complex) fails to undergo a functional transition ... [more]

Proc Natl Acad Sci U S A Sep. 22, 2015; 112(38);E5271-80 [Pubmed: 26351692]

Quantitative Score

  • 14.0 [Normalized Spectral Abundance Factor]

Throughput

  • High Throughput

Additional Notes

  • the maximum background-corrected normalized spectral abundance factor (NSAF) obtained from purifications of any of the bait variants is reported here
  • the prey protein was identified in at least one of purifications
  • wild type and several mutant versions of Alg-1 were affinity purified and associated proteins were identified by mass spectrometry

Curated By

  • BioGRID