BAIT

MLLT4

AF6
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
GO Process (4)
GO Function (2)
GO Component (6)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor.

Hock B, Boehme B, Karn T, Yamamoto T, Kaibuchi K, Holtrich U, Holland S, Pawson T, Ruebsamen-Waigmann H, Strebhardt K

Eph-related receptor tyrosine kinases (RTKs) have been implicated in intercellular communication during embryonic development. To elucidate their signal transduction pathways, we applied the yeast two-hybrid system. We could demonstrate that the carboxyl termini of the Eph-related RTKs EphA7, EphB2, EphB3, EphB5, and EphB6 interact with the PDZ domain of the ras-binding protein AF6. A mutational analysis revealed that six C-terminal ... [more]

Proc. Natl. Acad. Sci. U.S.A. Aug. 18, 1998; 95(17);9779-84 [Pubmed: 9707552]

Throughput

  • Low Throughput

Curated By

  • BioGRID