BAIT

HMG-3

CELE_C32F10.5, C32F10.5
HMG
Caenorhabditis elegans
PREY

TUFM-2

CELE_C43E11.4, EF-Tu2, C43E11.4
tufm-2 encodes the C. elegans ortholog of the mitochondrial TU elongation Factor (EF-Tu); loss of tufm-2 activity activates the mitochondrial unfolded protein response.
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and Human Cells.

Kolundzic E, Ofenbauer A, Bulut SI, Uyar B, Baytek G, Sommermeier A, Seelk S, He M, Hirsekorn A, Vucicevic D, Akalin A, Diecke S, Lacadie SA, Tursun B

The chromatin regulator FACT (facilitates chromatin transcription) is essential for ensuring stable gene expression by promoting transcription. In a genetic screen using Caenorhabditis elegans, we identified that FACT maintains cell identities and acts as a barrier for transcription factor-mediated cell fate reprogramming. Strikingly, FACT's role as a barrier to cell fate conversion is conserved in humans as we show that ... [more]

Dev Cell Dec. 10, 2017; 46(5);611-626.e12 [Pubmed: 30078731]

Throughput

  • High Throughput

Additional Notes

  • bait proteins were affinity purified and associated prey proteins were identified by mass spectrometry
  • score is p-value

Curated By

  • BioGRID