An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18.

Lin CI, Orlov I, Ruggiero AM, Dykes-Hoberg M, Lee A, Jackson M, Rothstein JD

Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L-glutamate/D,L-aspartate cell-membrane transport protein. It is expressed in brain as well as several non-nervous tissues. In brain, EAAC1 is the primary neuronal glutamate transporter. It has a polarized distribution in cells and mainly functions perisynaptically to transport glutamate from the extracellular environment. In the kidney it is involved in renal acidic ... [more]

Nature Mar. 01, 2001; 410(6824);84-8 [Pubmed: 11242046]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SLC1A1 ARL6IP5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lin CI (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SLC1A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

L-glutamate transmembrane transporter activity [IDA]cysteine transmembrane transporter activity [IDA]

Gene Ontology Cellular Component

ARL6IP5